Many pathologies and physiological conditions can be diagnosed through the analysis of blood plasma which provides crucial information of various internal organs. In order to fulfill these analysis most researchers, scientist and doctors use centrifugation to separate plasma from whole blood. But this technique requires a laboratory. In order to minimize error, reduce the time from blood collection to the test and provide faster and yet less expensive and comprehensive results, iBLOOD is an attractive technology for blood plasma separation and analysis. Accordingly, iBLOOD focuses on providing a a self-driven blood plasma separator as the first step toward the realization of single use, self‐blood test which now takes multiple technicians’ hours to do.
Hyprophilic characteristics of iBLOOD materials
One of the characteristic features of iBLOOD is that it works automatically just by capillary pressure which eliminates the need of external sources. The requested capillary pressure to drive the sample depnds on the material hydrophilicity and surface tension, iBLOOD is manufactured using hydrophilic, biocompatible porous materials that allow plasma extraction without manipulation.
Novel design of iBLOOD capillary pumping methodology
The core design of iBLOOD takes advantage of a novel design of capillary pump( a MIMP -Microchannel Integrated Micropillars). In this step, the hydrodynamic effect of different MIMP shapes with low aspect ratio (H/D ranged from 0.06 to 0.2) not only improve and optimize the design of microcapillary pumps for different passive microfluidic applications but also in particular to maximize the throughput.
Microfluidics in the iBLOOD technology
Finally, iBLOOD can combine microfluidics with conventional lateral flow immune chromatography to extract enough plasma to perform a blood panel. The iBLOOD design is a combination of cross-flow filtration with a reversible electroosmotic flow that prevents clogging at the filter entrance and maximizes the amount of separated plasma. The main advantage of iBLOOD is its efficiency, since with a small amount of sample (a single droplet ~10µL) a considerable amount of plasma (more than 1µL) is extracted and collected with high purity (more than 99%) in a reasonable time (5 to 8 minutes).
iBlood, a clinical tool! iBlood has been combined with lateral flow immune chromatography technology obtaining the same quality of results with less sample and without dilution. The picture shows a qualitative detection of the TSH (thyroid-stimulating hormone).
iBLOOD for measuring thyroid stimulation hormone levels.
This project has been partially supported through the call for Innovation projects with potential to be incorporated in the production sector (ACOSTA'T AL MERCAT 2020) and 50% is supported from Fons Europeus de Desenvolupament Regional de la Unió Europea in the Program FEDER de Catalunya 2014-2020.
- Hojjat Madadi, Jasmina Casals-Terré “A self driven microfluidic device for separating liquid from a liquid including deformable particles” W2015/071515A1. (To license email: Jasmina.firstname.lastname@example.org)
- Madadi H., Casals-Terré J. (2013) “Long-term behavior of nonionic surfactant-added PDMS for self-driven microchips” Microsystem Technologies, 19 ( 1), pp 143-150. Impact Factor:0.827
- Madadi H., Mohammadi M., Casals-Terré J., Castilla-López R, (2013) “A novel fabrication technique to minimize PDMS-microchannels deformation under high-pressure operation” Electrophoresis 34(22), DOI:10.1002/elps.201300340. Impact Factor: 3.261
- Madadi H., Casals-TerréJ., Castilla-López R., Sureda M. (2013) “High throughput microcapillary pump with efficient integrated low aspect ratio micropillars” Microfluidics and Nanofluidics, 17(1) DOI:10.1007/s10404-013-1295-5. Impact Factor: 3.218
- Madadi H., Casals-Terré J., Mohammadi M. (2015) ” Self driven efficient blood plasma separator microfluidic chip for point of care testing“ Biofabrication Journal 7(2). Impact Factor: 4.302
- Mohammadi M., Madadi M., Casals-Terré J., Sellarès J. (2015) “Hydrodynamic and Direct-current insulator-based dielectrophoresis (H-DC-iDEP) microfluidic chip for blood plasma separation “Analytical and Bioanalytical Chemistry 407(16) pp. 4733-4744. Impact Factor: 3.58
- Mohammadi M., Madadi M., Javad Zare M., Casals-Terré J., (2016) “A new approach to design an efficient micropost array for enhanced direct-current insulator-basedielectrophoretic trapping “Analytical and Bioanalytical Chemistry. Impact Factor: 3.58
S Karimi, P Mehrdel, J Farré-Lladós, J Casals-Terré -(2019) A passive portable microfluidic blood–plasma separator for simultaneous determination of direct and indirect ABO/Rh blood typingLab on a Chip, 2019 Impact factor: 6.914
- S Karimi, P Mehrdel, J Casals-Terre, J Farré-Lladós (2020) "Cost-effective microfabrication of sub-micron-depth channels by femto-laser anti-stiction texturing" Biofabrication. 2020 Feb 26;12(2):025021. doi: 10.1088/1758-5090/ab6665. Impact Factor: 7.236